Technique boosts omega-3 fatty acid levels in brain, improves cognitive function

While supplements containing beneficial omega-3 fatty acids like eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) exist, there is little evidence that they adequately increase EPA and DHA in the brain. According to researchers, a person would need to consume a small glass of EPA every day to effectively increase levels in the brain.

Now, new research from the University of Illinois at Chicago, published in the Journal of Lipid Research, shows that adding a lysophospholipid (LPC) form of EPA to the diet can increase levels in the brain 100-fold in mice.

The researchers gave mice a small amount of LPC-EPA, less than a milligram per day. The human equivalent would amount to less than a quarter of a gram per day, they say.

The omega-3 fatty acids DHA and EPA are known to have anti-inflammatory effects and protect against various neurological and metabolic diseases. DHA has been shown to be good for memory and cognitive deficits associated with Alzheimer's disease, and in pre-clinical studies, EPA has been shown to be effective in treating and preventing depression.

DHA is already prevalent in the brain, and there is little evidence to support the idea that eating lots of fish oil, either through whole fish or supplements, increases levels of DHA in the brain. EPA is found in very low concentrations in the brain. Boosting EPA levels in the brain through consuming EPA has proven difficult because the amount of EPA that would need to be ingested to show increases in brain EPA levels is quite large, 40 to 50 milliliters daily. Researchers still don't really have a great understanding of how EPA works to reduce depression and how much is needed in the brain to have these anti-depressive effects.

"In order to do the trials to determine the proper dosage and how EPA works in regards to depression, we need to have a better way of getting it into the brain because you need to consume so much of it that it’s just not practical, at least for human trials," said Papasani Subbaiah, PhD, co-author and professor of medicine and biochemistry and molecular genetics in the UIC College of Medicine.

Subbaiah reports that providing EPA in the form of lysophospholipid, unlike the type present in fish oil supplements, escapes degradation by pancreatic enzymes, which render it unable to pass into the brain.

"It seems that there is a transporter at the blood-brain barrier that EPA must pass through in order to get into the brain, but EPA in fish oil can't get through, whereas LPC-EPA can," Subbaiah said. "You don't have to consume all that much LPC- EPA to have significant increases of EPA show up in the brain, so this could be a way to do rigorous studies on the effects of EPA in humans.”

Producing LPC-EPA is not difficult, and it can be incorporated into feed pellets that Subbaiah fed to laboratory mice. These mice had up to 100 times more EPA in their brains after eating one milligram per day of the LPC-EPA in their feed for 15 days than mice eating plain EPA. Interestingly, the mice eating LPC-EPA also had two times more DHA in their brains.

"This study is proof of the concept that we can increase levels of both EPA and DHA in the brain via supplements or by incorporating LPC-EPA in the diet," said Subbaiah. "Using this technique, we can now perform critical studies to see if increasing concentrations of these fatty acids in the brain can help prevent and treat Alzheimer's and depression in mouse models, and then move into human trials if results are promising."